《植物生理学报》 2012, 48(12): 1141-1150

基因工程的技术飞跃——TALE技术的研究进展

李季, 韩宝达, 崔国新, 李立新*

东北林业大学盐碱地生物资源环境研究中心, 东北油田盐碱植被恢复与重建教育部重点实验室, 哈尔滨150040

通信作者：李立新；E-mail: lixinli1@gmail.com；Tel: 0451-82192237

摘　要：

类转录激活因子(TALEs)是一类来源于植物致病菌黄单胞菌属(Xanthomonas)的III型效应因子。TALE由N端转移结 构域、串联重复的DNA结合结构域、C端核定位信号及酸性转录激活结构域组成。TALE的DNA结合结构域经基因工程 修饰能够识别并结合任意指定的DNA序列, 从而可以实现对目的基因组位点的调控。目前, 已有多种TALE的快速构建 法。因此, TALE以及TALE衍生蛋白在基因工程领域具有广阔的应用前景。本文综述了TALE的研究现状, 对TALE技术的 优点和不足进行了简要的阐述, 并对其应用前景做了初步探讨。

关键词：TALE分布; TALE结构特征; TALE构建方法; TALE应用前景

收稿：2012-10-12   修定：2012-12-07

资助：中央高校基本科研业务费专项资金(DL09DA02)、国家自然 科学基金(30970223)和中国博士后科学基金(200902365)。

Advances in TALE Research

LI Ji, HAN Bao-Da, CUI Guo-Xin, LI Li-Xin*

Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field of Ministry of Education, Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China

Corresponding author: LI Li-Xin; E-mail: lixinli1@gmail.com; Tel: 0451-82192237

Abstract:

Transcription activator-like effectors (TALEs) are type III effector proteins from plant-pathogenic bacteria of the genus Xanthomonas. All TAL effectors are composed of an N-terminal translocation domain, central tandem repeat DNA binding domain, C-terminal nuclear localization signals and an acidic transcriptional activation domain. Central repeats of TALE can be engineered to bind any user-selected DNA targets. Consequently, TALE proteins can be engineered to achieve site-specific modification in the genome. Recently, several rapid TALE assembly approaches have been established. Therefore, TALE and TALE-derived proteins could be widely used for genome engineering. This article reviews recent advances in TALE research.

Key words: distribution of TALE; structural features of TALE; TALE assembly approaches; prospect of TALE